수업
협동과정 뇌과학전공 초청 세미나 6.1(목) (연자: 조용철 DGIST 교수님)
2023-05-30
- 제목 : Ribosomal protein-mRNAs are an axonal injury-responsive regulon for promoting regeneration under Gpr151 mRNA and Csde1 protein control
- 연자 : 조용철 교수님 (DGIST 뇌과학과)
- 초정자 : 허은미 교수님 (서울대학교 수의학과)
- 일시 : 2023년 6월 1일 (목) 오전 11시
- 장소: 서울대학교 관악캠퍼스 28동 101호
- Abstract :
New protein synthesis is crucial for axon regeneration after injury, which is mediated by mTOR pathway. However, the injury-related metabolism of ribosomes, the core complex of translation is not understood. We found that ribosome protein-coding mRNAs (RP-mRNAs) are a novel regulon under control of an RNA-binding protein, cold shock domain-containing E1 (CSDE1) that regulates translation and stability of its binding RNA. The binding between RP-mRNAs and CSDE1 protein become stabilized in mouse dorsal root ganglion neurons after sciatic nerve injury, which requires Gpr151 mRNA that is identified by the analysis of differential gene expression plus ribosome association efficiency. Gpr151 mRNA highly increases but does not direct to ribosomes for its translation. Instead, injury-induced Gpr151 mRNA binds to CSDE1 via its 5’-untranslated region (5’UTR) and increases the stability of the interaction between CSDE1 and RP-mRNAs. Gpr151-mediated stabilization is required for directing RP-mRNAs regulon to ribosomes. This pathway promotes axon regeneration in vitro and in vivo in sciatic and optic nerve after injury, indicating that 5’UTR of Gpr151 is a potential tool for developing therapeutic applications to improving axon regeneration via enhancing ribosomal association and stability of RP-mRNAs.